MyBioSource.com's Anti-Rat CD45RA/B PE (Clone OX-33) (mouse IgG1) is a Mouse monoclonal antibody. It reacts with Rat. This antibody has been shown to work in applications such as: Immunohistochemistry.
Description
Rat Leukocyte Common Antigen has been shown to exist in different forms on different lymphoid cell types. Rat L-CA on thymocytes shows one main band at 180 kDa, T cells show 4 bands at 180, 190, 200 and 220 kDa. MBS520157 detects a subfraction of the 240 kDa rat L-CA band found only on B lymphocytes. This monoclonal antibody is of particular use in the investigation of the molecular and antigenic heterogeneity of rat L-CA, especially when used in conjunction with other OX antibodies. Four mouse monoclonal antibodies (MRC OX 1, 28, 29 and 30) react with all molecular forms of L-CA and fall into two sets that are noncompetitive in binding to L-CA (MRC OX 1, 28, 29 and 30). The antigenic determinants seen by all these antibodies are lost when L-CA is reduced and alkylated. Three antibodies (MRC OX 22, 31, and 32) react selectively with B cells, T cytotoxic cells and about two thirds of T helper cells. OX 22 and OX 31 compete for binding but are non- competitive with OX 32. All of these antibodies bind to a sub-fraction of the 190, 200 and 220 kDa forms of T cell L-CA but not at all to the 180 kDa form of T cells or thymocytes